INNV-14. PROSPECTIVE SURGICAL STUDY ON THE FEASIBILITY OF SEMI-AUTOMATED TISSUE COLLECTION, STABILIZATION, PRESERVATION, AND SITE TRANSFER IN GLIOBLASTOMA - IMPROVING UNDERSTANDING OF IMMUNE LANDSCAPE OF TUMOR

Abstract INTRODUCTION Despite advances in targeted therapy and immunotherapy of many cancers, glioblastoma outcome is still suboptimal part due to heterogeneity tumor. Standard surgical approach for remove the enhancing tumor adjuvant treatment used treat residual infiltrative non-enhancing wall. We evaluated samples from both areas using advanced techniques intraoperative resecting, collecting biologically preserving viable fresh tissue evaluation immune landscape special genomics METHODS 20 patients with enrolled study. Before removal tumor, 3 different central core, rim portion were obtained navigation assisted NICO Myriad. Fresh was collected separately by an intraopertive Automated Tissue Preservation System (ATPS) promptly prepare iced-cold container laboratory viability, bio-banking flow-cytometric analysis. RESULTS Average age 65 years-old 11 female (55%). No adverse event observed on average, sampling added 10 minutes time. Flow-cytometry showed that compared has less cells abundance multiple subsets including lower CD8:CD4 ratio more abundant CD4 memory T-cells. However there remain activated/exhausted T-cells suppressive myeloid sent micro-RNA analysis next-generation sequencing as well. CONCLUSION Taken together, semi-automated collection, stabilization, preservation ATPS a safe feasible technique obtain glioblastoma, especially This information can be developing therapy. Initial results flow-cytometry overall decreased leukocyte infiltration, underrepresentation selected cell portion. Further studies regarding are underway.